Abstract
Two different type of soil sample was collected.Fish market soil sample was collected from Patna(Bihar).Vermi compost soil sample was collected from Varanasi.Both soil sample were serial diluted and plated on nutrient agar medium.Identification and characterization of Bacillus species was performed by different tests.Three different media were used.Soyabean, green peas and rice bran were used as the natural media.Nutrient broth was used as the synthetic media.Pure culture was isolated for the production of protease enzyme by Bacillus species.The secretion of protease enzyme was achieved by three natural media and one synthetic media(nutrient broth).Among the two different soil sample Vermi -compost soil sample was proved to be better than the fish market soil sample.In the case of natural media green peas shows the better enzymatic activity followed by the rice bran and soya bean. The optimum temperature and pH for the production of protease enzyme was analyzed on 40 ˚C and 8.0 respectively. Protease enzymes produced by the Bacillus species is widely used in the various industries like leather,food,detergent and pharmaceutical industries.
