Abstract
A new simple, sensitive reverse phase high performance liquid chromatography method was developed for the estimation of Trimetazidine in pharmaceutical dosage form. In this method, an Altima C-18 column (4.6 x 150mm, 5μ particle size) was used as stationary phase and Phosphate buffer: acetonitrile (90:10v/v) was used as mobile phase at a flow rate of 1.0 mL/min. The column temperature was maintained at 30°C and the detection was carried out using a PDA detector at 233 nm. Validation parameters such as system suitability, linearity, precision, accuracy, specificity, limit of detection (LOD), limit of quantification (LOQ), Stability of sample and standard stock solutions and robustness were studied as reported in the International Conference on Harmonization guidelines. The retention time for Trimetazidine was 3.2 min. The method was fast, accurate, precise and sensitive hence it can be employed for routine quality control of Trimetazidine tablets in quality control laboratories and pharmaceutical industries.
