Abstract
A simple, rapid, precise, accurate RP-HPLC method has been developed and validated for the simultaneous estimation of Lamivudine, Zidovudine And Nevirapine in tablet dosage form. Chromatographic separation was achieved with mobile phase consisting of Acetonitrile, Methanol and Phosphate Buffer pH 5.0±0.3 in the ratio of 20:30:50 v/v with Waters symmetry C18 (250 × 4.6 mm × 5 µm), column at a flow rate of 1 mL/min and detection wavelength was 246 nm. The retention times of Lamivudine, Zidovudine And Nevirapine were found to be 3.085 min, 3.680 and 4.830 min respectively. The linearity range for Lamivudine, Zidovudine And Nevirapine were found to be 30-70 µg/mL, 60-140 µg/mL and 39.9-93.2 µg/mL, respectively. The coefficient of correlation, (R2) for Lamivudine, Zidovudine And Nevirapine were found to 0.999, 0.999 and 0.999, respectively. The method was validated as per the ICH guidelines and can be employed for routine quality control analysis.
