Abstract
A rapid, specific and accurate high performance liquid chromatographic method for the determination of propronolol in K2EDTA human plasma using tramadol as internal standard was developed and validated using an Electrospray Ionization (ESI)-mass spectrometry detection. The extraction process involved a solid phase extraction. Both propronolol and the internal standard were eluted under isocratic mode using a Inertsil 50 X 4.6 mm i.d, 5 µm column. The mobile phase composed a mixture of 10:90 % v/v 5mM ammonium acetate solution and acetonitrile at a flow rate of 0.6 mL/minute. The injection volume is 5 µL. The runtime of the method is 2 minutes. The method showed good linearity in the range of 1.000 – 402.171 ng/mL. The mean recovery of propronolol from all the quality control samples is 65.83 % with a % coefficient of variation of 7.38 % and recovery of internal standard was 82.12% with a % coefficient of variation of 3.37 %. Matrix effects were not observed. The method is validated as per ICH guidelines.
