Abstract

Analytical methods are made to establish the identity, purity, physical characteristics and potency of the drugs. Methods may also support safety and characterization studies or evaluations of drug performance. Atorvastatin calcium (ATV) and Sitagliptin phosphate (STG) are usually co-administered and as there was no analytical method developed so far a new reverse phase analytical high-performance liquid chromatographic (HPLC) method for the simultaneous estimation of ATV and STG was developed. The proposed method was based on the separation of two drugs in reversed phase mode with the help of HiQ Sil C-18 HS (4.6mm ø 250 nm) analytical column. Both the drugs were detected with the help of UV detector at the wavelength of 255 nm. The current method was validated according to the ICH guidelines for Linearity, Accuracy and Precision, System stability, Detection and Quantification and Percent recovery etc.