Abstract

The paper presents the ring opening reaction of itaconic anhydride with 4-aminoantipyrine resulted in the formation of title compound, 4-[(1,5-Dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)amino]-2-methylidene-4-oxobutanoic acid (1). The structure of the newly synthesized compound was characterized by IR, ¹H NMR, ¹³C NMR, mass, elemental and thermal analysis data. The present study examines the possible antioxidant, antimicrobial and thermal properties of the titled compound 1. In order to evaluate the antioxidant activity, different in vitro methodologies such as DPPH radical scavenging, nitric oxide radical scavenging and reducing power assays were used. In addition, in vitro antimicrobial activity of the compound 1 was tested against four microorganisms. Antioxidant and antimicrobial activities of the compounds 1 were found to be significant in comparison with standard in all the in vitro methodologies adopted. Thermal stability of the compound 1 was determined by using TGA, DTA, DSC analysis  and the results showed that endothermic peak is correlate with the value (189–190 ^oC) measured by a capillary method.