Abstract

Niflumic Acid in human plasma using Diclofenac as an internal standard (IS) was determined quantitatively by a well developed and validated RP - HPLC method by UV detection. The extraction process involved a protein precipitation technique. Both Niflumic Acid and the internal standard were eluted under isocratic mode. The mobile phase composed of a mixture of 60:40 % v/v methanol and 10mM mixed phosphate buffer (pH 3.0±0.05) at a flow rate of 1.0 mL/Minute. The wave length of detection is 295 nm. The injection volume is 20 µL and the runtime of the method is 7.5 minutes. The method showed good linearity in the range of 55.44 – 5082.41 ng/mL. The recovery of Niflumic Acid is 7.79 % with a standard deviation of 0.587 and recovery of internal standard was 9.39 %. The LOD of Niflumic Acid is 59.14 ng/mL. Matrix effects were not observed.