Abstract

A simple, rapid, and precise method was developed for quantitative analysis of Lornoxicam (LXM) in bulk as well as in pharmaceutical dosage forms. SUMMARY:  The chromatographic separation of LXM was achieved on a Hypercil BDS C₁₈ (4.6 x 150mm, 5 mm) analytical column with Potassium Dihydrogen Phosphate Buffer & Acetonitrile [HPLC Grade] in the ratio of 40:60 (v/v), as mobile phase at ambient temperature. The flow rate was 1.0 ml/min and detection was by absorption at 382 nm using a PDA detector. The number of theoretical plates and tailing factor for LXM were found to be 1483.4 and 1.5 respectively. The linearity of the method was excellent over the range 10–50 µg/ ml LXM. The correlation coefficient was 0.9999. The relative standard deviations of peak areas from five measurements were always less than 2%. The proposed method was found to be suitable and accurate for quantitative analysis of LXM. CONCLUSION: The present work was undertaken with the aim to develop and validate a rapid and consistent RP-HPLC method development in which the peaks will be appear with short period of time as per ICH Guidelines. The proposed method was adequate sensitive, reproducible and specific for the determination of Lornoxicam bulk as well as in its Pharmaceutical dosage form. The validation of method was carried out utilizing ICH-guidelines. The described RP-HPLC method was successfully employed for the analysis of pharmaceutical formulations containing the dosage form. The proposed method was simple, fast, Accurate and precise method for the Quantification of drug in the dosage form, bulk drug as well as for routine analysis in Quality control. Overall the proposed method was found to be suitable and Accurate for the Quantitative determination and stability study of the drug in Pharmaceutical dosage form.