Abstract
The present study deals with simultaneous estimation of Eprosartan and Hydrochlorothiazide by RP-HPLC in tablet dosage form. The two drugs have anti-hypertensive activity. The present research work describes a rapid and accurate RP-HPLC method for simultaneous estimation of Eprosartan and Hydrochlorothiazide from the combination tablet dosage form. The separation of two drugs was achieved by on X-terra (4.6 mm X 150 mm i.d) 5μ column. The mobile phase consists of 0.1% OPA (3.5 pH): Methanol: Acetonitrile (60:20:20). The detection was carried out at a wavelength 230 nm. The method was validated for system suitability, linearity, accuracy, precision, robustness and stability of sample solution as per ICH guidelines. The linear ranges for Eprosartan and Hydrochlorothiazide were 50-300μg/ mL, 2.5-12.5μg /mL respectively with good recovery i.e., 100.3% to 99.8%.
