Abstract
Tirofiban hydrochloride is an anti-platelet agent. Three methods a UV-spectroscopic method, HPTLC method and RP-HPLC methods were developed for the estimation of tirofiban hydrochloride in pharmaceutical formulation. In UV method the sample was dissolved in methanol and measurements were made at 226nm. HPTLC separation was carried out with solvent system hexane: methanol: acetic acid (5:3.5:0.5% v/v) on a silica gel G60F254 plate. The Rf value of tirofiban was found to be 0.46. In RP-HPLC the separation was carried out by C18 column using mobile phase buffer (sodium dihydrogen phosphate at pH-5): acetonitrile in the ratio of (70:30% v/v). Linearity for the methods was found to be 10-30µg/ml, 3-18ng/spot and 600-1400ng/ml, respectively for UV, HPTLC and RP-HPLC methods. All the three methods were validated according to ICH guidelines and successfully employed for estimation of tirofiban hydrochloride from formulation. The estimated amount was well in conformation with the label claim. All The three methods reported here are simple, accurate, sensitive and cost effective in estimation of tirofiban from pharmaceutical formulation.
