Abstract
A new, precise, rapid, accurate RP-HPLC method was developed for the Simultaneous Estimation of Miglitol and Metformin in tablet dosage form. After optimization the good chromatographic separation was achieved by Isocratic mode with a mixture of Phosphate Buffer (pH 6.5):methanol:acetonitrilein the ratio of 50:30:20 v/v/v as the mobile phase with , column as stationary phase at flow rate of 1 mL/min and detection wavelength of 210 nm. The retention times Metformin and Miglitol found to be 2.420min and 3.580 min respectively. The linearity of this method was found in the concentration range of 60 μg/mL to 140 μg/mL for Metforminand 3μg/mL to 7μg/mL for Miglitol. The correlation coefficient R2 value is found to be 0.997for metformin and 0.9973 for Miglitol. The LOD and LOQ for Metformin were found to be 2.79ppm and 8.45ppmrespectively. The LOD and LOQ for Miglitol were found to be 5.23 ppm and 15.83 ppm respectively. This method was found to be good percentage recovery Metformin and Miglitol were found to be 100.67 and 99.54 respectively indicates that the proposed method is highly accurate.
