Abstract
Three simple and sensitive first order derivative spectrophotometric methods (D1) were developed for the determination of Azacitidine in pharmaceutical formulations using water (Method A), phosphate buffer pH 4.0 (Method B) and phosphate buffer pH 5.0 (Method C). Beer’s law was obeyed over concentration range 1-30 μg/mL for both Method A, B and C respectively. The linear regression equations were found to be y = 0.0063x - 0.0025, y = 0.0057x - 0.003 and y = 0.0073x - 0.0033 for Method A, B and C respectively. The three methods were validated and no interference of the excipients was observed.
