Abstract
A simple, precise, accurate and economical high performance thin layer chromatographic (HPTLC) method has been developed for the simultaneous estimation of Tramadol Hydrochloride and Diclofenac Sodium in bulk and formulation. The separation was carried out on Merck TLC aluminum sheets of silica gel 60F254, using Methanol: Ethyl acetate: Chloroform: Toluene (4:2:2:2, v/v/v/v) as mobile phase and densitometric analysis of compound was carried out in absorbance mode at 273 nm. The linear regression analysis data for the calibration plots for TH and DS showed good linear relationship with regression coefficient (r2) of 0.996 in the concentration range of 1000–4500 ng/spot for TH and DS both. The method was validated for specificity, linearity, accuracy, precision and robustness as per the ICH guidelines. The method was successfully applied for simultaneous estimation of TH and DS from bulk and tablet dosage forms.
