Abstract

A simple efficient, precise and accurate spectroscopic method has been developed and validated for quantitative estimation of Rivastigmine tartrate in bulk and pharmaceutical dosage form. Rivastigmine tartrate is soluble in 0.1N HCl so it was used as solvent. Rivastigmine tartrate is dissolved in 0.1N HCl the resulting solution was then scanned in the UV range (200-400nm) in a 1cm quartz cell in a double beam UV spectrophotometer. The λmax of Rivastigmine tartrate was found to be 263.1 nm. The method obeys Beers law in the concentration range from 10-90 μg/ml. The correlation coefficient was found to be 0.999 (r2═ 0.999). The LOD and LOQ were found to be 2.68 and 8.12 μg/ ml respectively. The result of estimation of marketed formulation (Exelon) was found to be 99.02 %. The accuracy of the method was determined by recovery studies. The percentage recovery was found to be 99.9%. The method was validated statistically as per ICH guidelines. The method showed good reproducibility and recovery with % RSD less than 2. So, the proposed method was found to be simple, specific, precise, accuracy, linear, and rugged. Hence it can be applied for routine analysis of Rivastigmine tartrate in bulk drug and the Pharmaceutical formulations.