Abstract
An accurate RP-HPLC method with short retention and run times was developed for estimation of linagliptin in its tablet dosage forms. Good separation of the drug was achieved on an X Bridge C18 column (100 x 4.6 mm; 5m) by using a mobile phase consisting of a mixture of phosphate buffer (pH 3.4) and acetonitrile (70:30 v/v) at a flow rate of 1.0 mL/min. The drug in the eluates was monitored by UV detection at 240 nm. Under the optimized conditions, the retention time obtained for the drug was 2.791 min. The linearity of quantification was observed in the concentration range of 25-150 µg/mL of the drug. The validation of the method was done by following the ICH guideline. The proposed method could be applied for determination of linagliptin in its tablet dosage forms without any interference from normal excipients.
