Abstract
A simple, specific, precise, and accurate RP-HPLC method has been developed and validated for the estimation of Cabazitaxel in bulk and parenteral formulations. Chromatographic separation was achieved on Thermo Hypersil ODS C18(250 x 4.6mm x 3.5µ) column using potassium dihydrogen phosphate buffer (Adjusted PH 3.0 with Orthophosphoric acid) and Acetonitrile in the ratio 30:70 at a flow rate of 1.2 ml/min with UV detection at 223 nm.The retention time was around 4.6.Linearity was observed in the concentration range of 30-90 µg/ml (R2=0.999) with regression equation y = 26209x + 204.79.The Limit of Detection and Limit of Quantification were found to be 0.398 and 1.3274µg/ml respectively.
