Abstract
The aim of the present work was to develop and validate a simple, efficient, economical RP-HPLC method for the analysis of Atenolol (AT) in samples obtained from in vitro transdermal permeation studies. A Phenomenex C18 reverse phase column (150 x 4.6mm, 5µm) with mobile phase containing 10mM ammonium acetate: methanol (75:25% v/v) at a flow rate of 1.2mL/min was used at isocratic mode and eluents were monitored at 225nm. The retention time of AT was 2.5min and showed a good linearity in the concentration range of 0.2-3µg/mL with a correlation coefficient >0.999. In vitro skin permeation studies were carried out using a Franz diffusion Cells. Absence of interference peaks at the retention time of AT indicates that the method was specific for the analysis of AT in samples obtained from in vitro transdermal permeation studies. The percent recoveries were ranged in between 98-102 (RSD < 2). The validation parameters like specificity, linearity, accuracy and limit of detection, limit of quantification, precision, robustness are all fulfilled regulatory requirements. The developed HPLC method could be successfully used for the quantification of AT in samples obtained from in vitro skin permeation studies.
