Abstract
A simple, selective and economical bioanalytical method was developed and validated for estimation of Quetiapine fumarate, an antipsychotic drug using High Performance Thin Layer Chromatography from human plasma. Drug was separated from plasma using liquid-liquid extraction technique and quantitated by High performance thin layer chromatography using Zolpidem tartrate as an internal standard. Separation was performed on Silica gel 60 F254 precoated aluminium plates as stationary phase with mobile phase consisting of Ethyl acetate: Toluene: Methanol (7:2:2 v/v/v). Densitometric detection was performed at 292nm. The method was found to be linear in the range of 300ng/ml to 8700ng/ml. The method was validated for linearity, accuracy, precision, recovery and selectivity and the results obtained were found to be within acceptable limits. This simple and selective method can be used for accurate and precise determination of Quetiapine fumarate in clinical samples and can be applied for Therapeutic drug monitoring and pharmacokinetic studies.
