Abstract
A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for the estimation of tinidazole in pure and pharmaceutical dosage forms. A Thermohypersil Symmetry C18 column (250 x 4.6mm x 5 µ) was used as a stationary phase with a mobile phase containing a mixture of acetonitrile and water in the ratio of 60:40v/v. The flow rate was 1.0ml/min, effluent was monitored at 295nm and eluted at 2.857min. Calibration curve was plotted with a range from 10-70µg/ml for tinidazole and the correlation was found to be 0.9996.The accuracy range was found between 99.52% and 100.05%. The %RSD values for both intraday and interday precision were less than 1%. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.42μg/ml and 1.27μg/ml respectively. The assay was validated for the parameters like specificity, system suitability, precision, accuracy, robustness and ruggedness parameters. The proposed method can be useful for the routine determination of tinidazole in pharmaceutical dosage form.
