Abstract
Soy derived, non-steroidal Phytoestrogenic compound Genistein is able to induce reproductive problems in mammals. In this research the effects of genistein on male mice with specific interest on semen quality and the hormonal milieu of gonadotropins and testosterone has been determined. Adult male mice received genistein (10mg/kg body weight/day) dissolved in 1:4 DMSO: PBS (vehicle) for 30, 60 and 90 days along with mice feed and water ad libitum. Control mice were kept on feed and water ad libitum and dosed with vehicle only. Post sacrifice sperm count, motility, morphology and sperm vitality index (SVI) were analyzed using cauda epididymal sperm suspensions and serum level LH, FSH and testosterone (T) levels were estimated opting appropriate ELISA techniques. Results showed significant (p < 0.01 to 0.001, via two way ANOVA analyses) chronological decrease in sperm count and motility along with increase in abnormal as well as dead sperms (Sperm Vitality Index, i.e., Live : Dead sperm ratio). LH, FSH and testosterone hormone levels also decreased chronologically throughout the experiments indicating male reproductive impairment due to prolonged intake of genistein. These effects are either directed on the testes or mediated via the hypothalamo-hypophysial gonadal axis.
