Abstract
Simultaneous determination of Voglibose and Metformin HCl has been accomplished using a high performance liquid chromatographic method with UV detection on C18 columns. Separation was achieved on a Inertsil ODS (150mm×4.6 mm) and The column was equilibrated with mobile phases consisted of acetonitrile: 0.01M phosphate buffer pH 3 (85:15, v/v) and The flow rate was 0.8 ml/min. and the total elution time was 10min. The selected chromatographic conditions were found to effectively separate Voglibose and Metformin HCl with retension time 2.11 ± 0.015 and 4.62 ± 0.020 min. Voglibose was derivatized by using Taurine and Sodium Periodate. This method was applied to combination of standard bulk drug and marketed formulations. The linearity range was found to be 0.01-0.06µg/ml and 10-60µg/ml for Voglibose and Metformin HCl respectively. The proposed method was found to be accurate, precise, reproducible and specific and it can also be used for routine quality-control analysis of these drugs in combination tablets.
