Abstract

This paper reports a validated RP-HPLC method for the simultaneous estimation of cetrizine hydrochloride and diethyl carbamazine in tablet dosage forms. The method is achieved on a phenomenex C18 (4.6 x 250mm, 5µ i.d) column, aided by a mobile phase mixture of methanol:pH 6 phosphate buffer in a ratio of 80:20 % v/v, at a flow rate 1ml/min and eluents were monitored at 231 nm. The average retention time of cetirizine and diethyl carbamazine were found to be 4.897 min and 3.797 min, respectively with the linear calibration curves between the concentration range of 0.20-0.70 mg/ml for CTZ and 0.5-3.0 mg/ml for DEC.  The correlation coefficient was found nearer to 0.999 with % RSD less than 1%. Number theoretical plates were 11842 and 11769 for CTZ and DEC, respectively. The percentage recovery was about 99.8±0.5% for both drugs. The method was proved to be more accurate, precise and rapid by statistical validation and recovery studies.