Abstract
This paper reports a validated RP-HPLC method for the estimation of diclofenac sodium and famotidine in combination formulation. The method is achieved on a phenomenex C18 column (4.6 x 250mm, 5µ i.d), using methanol: pH 6.8 buffer (85:15%, v/v), as mobile phase mixture, applying a flow rate of 1ml/min and eluents were monitored at 230 nm with an average retention time for diclofenac sodium and famotidine at 3.32 and 4.12 min, respectively and the linear calibration curves between the concentration range of 20-200 µg/ml for diclofenac sodium and 40-120 µg/ml for famotidine. The correlation coefficient was found nearer to 0.9998. The LOD and LOQ for diclofenac sodium was 0.04; 0.14 µg/ml and famotidine was 0.085; 0.272 µg/ml, respectively. The number of theoretical plates was found to be 21342 for diclofenac sodium and18769 for famotidine. The developed method was evidenced to be more accurate, simple, precise and rapid by statistical validation and recovery studies.
