Abstract

To separate and characterize the compounds from methanolic extract of C. guianensis flower using Liquid chromatography- Mass spectroscopy. The compound with the retention time 1.285 exhibited a pseudo molecular ionic peak at m/z 285 for the [M+H]⁺ may be identified as 5, 7-dihydroxy-6,8-dimethyl-2-phenyl chroman-4-one (1). The compound with the retention time 2.70 exhibited fragmented ionic peak at m/z 143.0, 243.0, 284.2 and 357.3 may be identified as 2''-O-pentosyl-8-C-hexosyl luteolin (2). The compound with pseudo molecular ionic peak at  m/z 285 for the [M+H]⁺ may be identified 5, 7-dihydroxy-6, 8-dimethyl-2-phenyl chroman-4-one and fragmented ionic peak at m/z 143.0, 243.0, 284.2 and 357.3may be identified as 2^''-O-pentosyl-8-C-hexosyl luteolin by using LC-MS technique. Only two compounds (1) and (2) were identified by LC-MS method. Since we have recorded only LC-MS we could able to identify a limited number of compounds which could have improved if we would have attempted for LC-MS/MS analysis.