Abstract
The aim of the present research work was to develop accurate and rapid third order Differential derivative spectrophotometric method. The stock solution was prepared by taking methanol as solvent. Further dilutions were made by using equi molar solutions with 0.1N NaOH and 0.1N HCl separately and the difference in absorptivities was measured at wavelengths of 214 nm. It was proved that spectrum differentiation significantly corrects errors resulting from overlapping background. Difference in absorbance between maxima and minima was calculated to find out the amplitude. The amplitude was plotted against concentration. Beer’s-Lambert’s law is valid in the concentration range of 30-50 μg/ml. The result of analysis was validated as per USP guidelines. The method was linear, accurate, precise, economic. This is the advanced method compared to absorbance maxima method.
