Abstract
A simple, Accurate, Precise and sensitive UV spectrophotometric method was developed for the simultaneous estimation of Paracetamol (PCM), Enalapril maleate (ENM) and Hydrochlorothiazide (HCTZ) in bulk and pharmaceutical dosage forms. This method employs solving of first order derivative spectroscopic method at the λ max values of PCM (278.60), ENM (250.80nm) and HCTZ(302.80).Calibration curves were linear in the range of 2-12 μg/ml for PCM and ENM and 4-24 μg/ml for HCTZ respectively. Recovery studies for PCM, ENM and HCTZ were performed and the percentage recovery for both the drugs was obtained in the range of 98.30-99.15 % and 98.5-99.27 % respectively. The method showed good reproducibility and recovery with % RSD less than 2. The proposed method can be successfully applied for the determination of PCM, ENM and HCTZ in pharmaceutical formulations and is validated according to ICH guidelines.
