Abstract
A simple, Accurate, Precise and rapid RP-HPLC method has been developed for estimation of Enalapril Maleate, (EM), Hydrochlorothiazide (HCT) and paracetamol in its pure and tablet dosage form. The mobile phase used mixed buffer consisting of(KH2PO4 and K2HPO4) and ACN in the ratio of (75:25%v/v) was delivered at the flow rate of 1.0 mL/min and detection was carried out at 210 nm. The separation was achieved using BDS C8 Hypersil column (Hi-Q 250x4.6mm ID; particle size 5µ m).The validation of method carried out as per ICH guidelines. The method was tested for linearity, accuracy, recovery study and specificity.Calibration curves were linear in the range of 10-60 for PCM, 5-30 ENM and 4-24 μg/ml for HCTZ respectively. Recovery studies for PCM, ENM and HCTZ were performed and the percentage recovery for both the drugs was obtained in the range of 98.59-99.35 % and 98.02-99.17 % respectively. The method showed good reproducibility and recovery with % RSD less than 2. The proposed method can be successfully applied for the determination of PCM, ENM and HCTZ in pharmaceutical formulations and is validated according to ICH guidelines.
