Abstract
A simple reverse phase liquid chromatographic method was developed and validated as per the ICH guidelines for the quantitative determination of Lornoxicam in pharmaceutical dosage forms. The method was simple, precise, specific and accurate. The mobile phase consists of pH 3.2 phosphate buffer, methanol and acetonitrile ( 3:1:1 v/v/v ). The proposed RP-HPLC method utilizes a 5μm Luna C18 phenomenex column (250 mm ×4.6 mm) at ambient temperature. The eluent was monitored at 273 nm and retention time of Lornoxicam was 3.910 min. The linearity was observed from 12.5-75μg/ml with r2= 1. The limit of detection and limit of quantitation were found to be 0.75μg/ml and 1.5μg/ml respectively. From the results obtained, it was worthwhile that the provided methods were robust for small and deliberate changes in experimental conditions.
