Abstract
RP-HPLC method is use for the determination of drugs in single or in combination with other component. RP-HPLC technique is widely use for the separation and analysis of drugs that is present in combined formulation. This technique is also used for the separation of drug from the excipients that are present in the formulation. Analytical method is developed to carry out the determination of drugs or component present in the formulation. Validation studies are performed in order to assess the validation parameters for analytical method developed in accordance to ICH guideline. In this article we can understand steps of method development and validation for the new drug or component by using RP-HPLC technique.
