Since a long time ago, one of the trendiest topics catching the attention of scientists is biosensing. This is true because biological systems are highly complex and are inextricably linked to the presence of a wholesome environment. Significant changes have also recently been made to the design of biosensors. Biosensors have gained popularity for a variety of uses, including the assessment of food quality, environmental monitoring, and the identification of clinical and metabolic issues. The field of nanotechnology has introduced several intriguing elements that have the potential to enhance sensing phenomena. Utilizing a variety of nanomaterials, including nanoparticles, nanotubes, nanorods, and nanowires, has greatly improved repeatability and allowed for speedier detection. The assembly of nanomaterial features is what gives rise to the special qualities of nanomaterials, which include strong electrical conductivity, improved shock resistance, sensitive reactions like piezoelectric and adaptable color-based detecting methods. This research focuses on the many kinds of biosensors that are based on various kinds of nanomaterials and discusses their implications and developmental features.

1. V. Bhardwaj and A. Kaushik, ―Biomedical applications of nanotechnology and nanomaterials,‖ Micromachines, vol. 8, no. 10, p. 298, 2017.

2. A. Kaushik, A. Yndart, S. Kumar et al., ―A sensitive electrochemical immunosensor for label-free detection of Zika-virus protein,‖ Scientific Reports, vol. 8, no. 1, p. 9700, 2018.

3. A. Kaushik and M. Mujawar, ―Point of care sensing devices: better care for everyone,‖ Sensors, vol. 18, no. 12, p. 4303, 2018.

4. A. Kaushik, A. Vasudev, S. K. Arya, S. K. Pasha, and S. Bhansali, ―Recent advances in cortisol sensing technologies for point-ofcare application,‖ Biosensors and Bioelectronics, vol. 53, pp. 499–512, 2014.

5. S. Vashist, ―Point-of-care diagnostics: recent advances and trends,‖ Biosensors, vol. 7, no. 4, p. 62, 2017.

6. S. K. Vashist, P. B. Luppa, L. Y. Yeo, A. Ozcan, and J. H. T. Luong, ―Emerging technologies for next-generation point-of-care testing,‖ Trends in Biotechnology, vol. 33, no. 11, pp. 692–705, 2015.

7. S. K. Metkar and K. Girigoswami, ―Diagnostic biosensors in medicine— a review,‖ Biocatalysis and Agricultural Biotechnology, vol. 17, pp. 271–283, 2019.

8. L. El Harrad, I. Bourais, H. Mohammadi, and A. Amine, ―Recent advances in electrochemical biosensors based on enzyme inhibition for clinical and pharmaceutical applications,‖ Sensors, vol. 18, no. 1, p. 164, 2018.

9. J. Wang, ―Electrochemical biosensors: towards point-of-care cancer diagnostics,‖ Biosensors and Bioelectronics, vol. 21, no. 10, pp. 1887–1892, 2006.

10. Ma C, Fan R, Ahmad H, Shi Q, Comin-Anduix B, Chodon T, et al. A clinical micro- chip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells. Nat Med. 2011;17(6):738-43.

11. Zhu H, Stybayeva G, Macal M, Ramanculov E, George MD, Dandekar S, et al. A microdevice for multiplexed detection of Tcell-secreted cytokines. Lab Chip. 2008;8(12):2197-205.

12. 10. Bandodkar AJ, Wang J. Non-invasive wearable electrochemical sensors: A re- view. Trends Biotechnol. 2014;32(7):363-71.

13. Abe K, Yoshida W, Ikebukuro K. Electrochemical biosensors using aptamers for theranostics. Adv Biochem Eng Biotechnol. 2014;140:183-202.

14. Du H, Strohsahl CM, Camera J, Miller BL, Krauss TD. Sensitivity and Specificity of Metal Surface-Immobilized ―Molecular Beacon‖ Biosensors. J Amn Chem Soc. 2005;127(21):7932-40.

15. Contreras-Naranjo JE, Aguilar O. Suppressing Non-Specific Binding of Proteins onto Electrode Surfaces in the Development of Electrochemical Immunosen- sors. Biosensors. 2019;9(1):15.

16. Arya SK, Park MK. 4-Fluoro-3-nitrophenyl grafted gold electrode-based platform for label free electrochemical detection of interleukin-2 protein. Biosens Bio- electron. 2014;61:260-5.

17. Chen Z, Sun M, Luo F, Xu K, Lin Z, Zhang L. Stimulus-response click chemistry- based aptamer-functionalized mesoporous silica nanoparticles for fluorescence detection of thrombin. Talanta. 2018;178:563-8.

18. Wu D, Liu Y, Wang Y, Hu L, Ma H, Wang G, et al. Label-free Electrochemilumi- nescent Immunosensor for Detection of Prostate Specific Antigen based on Aminated Graphene Quantum Dots and Carboxyl Graphene Quantum Dots. Sci Rep. 2016;6(1):20511.

19. Liu Q, Wu C, Cai H, Hu N, Zhou J, Wang P. Cell-based biosensors and their ap- plication in biomedicine. Chem Rev. 2014;114(12):6423-61.

20. Akshay P, et al. Pharmaceutical Biotechnology. PV Publication. 2020. 21. Chapman T, Legge C, Patel A. Utility of Biosensors in Pharmaceutical industry.Handbook of Biosensor and Biochips. 2008;3.