Disinfectants and antiseptics play a vital role in the infection control. In-use tests study was aimed to determine the contamination level in the commonly used disinfectants. A total of 160 samples were collected from the various departments. Ten milliliters (10 ml) sample of each disinfectant / antiseptic being used in the hospital was collected and processed by the method of Kelsey and Maurer (1974). Neutralization was done with the help of nutrient broth or nutrient broth with 3% v/v Tween 80. One milliliter of the disinfectant / antiseptic fluid was transferred to 9.0 ml of the diluents. A volume of 0.02 ml of this mixture was inoculated on to ten different areas of each of two well-dried nutrient agar plates. One plate was incubated at 37°C for 3 days and the other for 7 days at room temperature. Disinfectant and antiseptics was contaminated if they were growing five or more drops any plates. The organism thus grown was identified on the basis of colony morphology and biochemical reaction. In these 16 samples were failed in the test, out of 160 samples revealed the growth of Pseudomonas aeruginosa Staphylococcus sp.; Candidosis. In conclusion, all the disinfectants and antiseptics should be used in proper concentration and monitoring of disinfectants / antiseptics should be under taken on regular basis while they are being used.