An attempt has been made to evaluate the efficacy of different disinfectants and antiseptics being used in the various departments of Maharishi Markandeshwar Institute of Medical Sciences and Research, Mullana, District Ambala (Haryana). Of the total 100 samples of disinfectants and antiseptics tested 5(5%) have been found to contaminated of bacteria. The contamination arrived from the longer used, uncovered containers, prolonged use to the dilution without change, insufficient time allowed for the appropriate action and dipping in of dirty instrument constitute the other problem in the use of disinfectants / antiseptics and the reasons have been spell out.