Background: Water is essential in day-to-day life for every individual, drinking water supply has a primary objective of protecting human health, to ensure that the sufficient quantities of safe water is available across the globe. Most of the population in the world is facing scarcity of the safer water to drink and it majorly leads to the different water ion imbalances in the body and in the long run it affects the health severely and causes the various diseases. Aim: To assess the association between the quality of water and disease occurrence in the rural areas of Guntur. Objective: To monitor safety of water and secondary objective is assess the disease which is mostly prevailing due to lack of water quality and also create awareness on association between quality of water and disease occurrence. Methodology: A cross sectional study, people are screened based on inclusion and exclusion criteria and information had taken from the villagers by survey forms. The self-designed and validated questionnaire was used to access the data regarding the health issues like tooth discoloration, cardiovascular diseases, joint pains and central nervous system. Collected data was tabulated and interpreted using statistical software. Results: Tooth discoloration was associated with the fluorine concentration of daily usage water because in OBP (F-0.09mg/L). The composition of daily usage water is associated with the various diseases in Krosuru the usage water composition was not good because of that prevalence was high with various disease and disorders like joint related problems, CVS related etc. Conclusion: As a consequence of the aforementioned findings, the current study concluded that tooth discolouration was related to the fluorine content of water used on a daily basis (OBP; F-0.09mg/L). The composition of the water used on a daily basis in Krosuru is linked to a number of ailments, and as a result, the prevalence of numerous diseases and disorders, such as joint-related issues and CVS, was high.