Drug resistance is a serious and expanding threat to global public health, and it will take continuous and coordinated efforts to develop the novel range of antibiotics to battle against infectious diseases to address this problem. Many studies shown that the aniline and carbonyl compound derivatives have numerous biological and anti-bacterial activity. The purpose of this study was to create 2,3-Dichloroaniline derivatives and characterization of synthesized Schiff bases.1-H NMR and IR spectroscopic studies were used to assess the chemical structure of synthesized Schiff base. Also, it was determined whether the synthesized derivatives had antibacterial activity against Escherichia coli or Staphylococcus aureus. According to the findings, synthesized Schiff bases prevent both Escherichia coli and Staphylococcus aureus from growing. In closing, it's critical to note that the reagents utilized in this study are affordable and don't need additional handling instructions. All the synthesized Schiff bases shown satisfactory result in characterization and shown good zone of inhibition.