The antioxidant activity of a hydroalcoholic extract of polyherbal mixture (RVMP) using four different herbs, namely Rubia cordifolia, Vitex negundo, Piper nigrum, and Myristica fragrans, was determined in this study using 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay, hydroxyl radical scavenging assay, and nitric oxide scavenging assay (NO).The findings show that polyherbal mixture (RVMP) has significant antioxidant activity. The total phenolic content and flavanoids were also ascertained. The polyherbal mixture contained a significant amount of phenolic content. The current study's antioxidant activity data suggest that polyherbal mixture (RVMP) is a superior source of natural antioxidants, which may aid in the prevention of oxidative stress progression.The antioxidant activity of a hydroalcoholic extract of polyherbal mixture (RVMP) using four different herbs, namely Rubia cordifolia, Vitex negundo, Piper nigrum, and Myristica fragrans, was determined in this study using 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay, hydroxyl radical scavenging assay, and nitric oxide scavenging assay (NO).The findings show that polyherbal mixture (RVMP) has significant antioxidant activity. The total phenolic content and flavanoids were also ascertained. The polyherbal mixture contained a significant amount of phenolic content. The current study's antioxidant activity data suggest that polyherbal mixture (RVMP) is a superior source of natural antioxidants, which may aid in the prevention of oxidative stress progression.