Objective: the objective of study was standardizing single Unani herbs used in Polyherbal formulation. In this article, we have provided standardized value of specimen of Nakhood (Cicer arietinum), Baqila (Vicia faba), Turmus (Lupinous albus), Khatmi (Althea officinalis) and Methi (Trigonella foenum). Method: These drugs were assessed on physico-chemical and analytical parameters viz: 1) macroscopic and microscopic features, 2) extractive values 3) moisture contents 4) ash values 5) loss of weight on drying 6) pH of 1% and 10% solution. Results and discussion: Results showed all the drugs involved in study were of standard quality as there moisture content less than 10%, total ash value less than 10%, water-soluble ash values of the individual drugs were in the range of 0.8 to 3% w/w which shows all drugs were of good quality. Acid insoluble ash up to 1% and pH of 1% and 5% of each drug was found to be around 7 with mild basic in nature which is good to neutralize acidic medium produced on scalp because of dandruff. Extraction values done in aqueous medium shows water soluble constituents such as tannins, sugars, plant acids and mucilaginous compounds. Alcohol soluble extraction values shows the presence of tannins, alkaloid and resins similarly ether/ chloroform extraction value show presence of volatile oils, fixed oils and resins. Conclusion: polyherbal formulation is standard quality and its application for desired action can give promising results.