Antibiotic resistance is considered one of the world's major public health concerns. The main cause of bacterial resistance is the improper and repeated use of antibiotics. To alleviate this problem, new chemical substances against microorganisms are being synthesized and tested. Thiazolidines are compounds having many pharmacological activities including antimicrobial activities. For this purpose some thiazolidine derivatives substituted at position 5 in the thiazolidine nucleus were synthesized and tested against several microorganisms. From these reviews we synthesized a new series of 2-(4-chlorophenyl)-3-{4-[6-(substitutedphenyl)-2-thioxo-1,2,5,6-tetrahydropyrimidin-4-yl]phenyl}-1,3-thiazolidin-4-one derived from 3-{4-[3-(substitutedphenyl)prop-2-enoyl]phenyl}-2-(4-chlorophenyl)-1,3-thiazolidin-4-one and thiourea. The title compounds were characterized by element analysis, IR, NMR and spectral data. All the compounds were tested for their antibacterial and antifungal activities by Cup Borer method.