The objective of the present study was to determine the in-vitro antioxidant activity of aqueous extract of Albizia stipulate Boiv. bark. The antioxidant properties were evaluated by determining its free radical scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2’-azino-bis (3-ethyl benzothiazoline-6-sulfonic acid) (ABTS), and Superoxide ion. The aqueous extract possessed a12.52µg/ml for DPPH radical scavenging assay on comparing to standard Vitamin C (3.59µg/ml), an IC50 value of 3.39 µg/ml for ABST radical scavenging assay when compared to vitamin C (2.32µg/ml), and for Superoxide ion scavenging the IC50 value was recorded as 13.60?g/ml on compared to standard gallic acid (0.61 µg/ml). From the results in terms of radical scavenging activity against DPPH, ABTS, and Superoxide ion, Albizia stipulate Boiv. bark exhibited excellent antioxidant activity than standards. Hence, an aqueous extract of Albizia stipulate bark can be suggested for usage as an effective natural antioxidant in pharmaceutical and nutraceutical products.