Background: Breast cancer is the most common invasive cancer in women, and the second main cause of cancer death in women, after lung cancer. Traditional medicine has been used to treat various types of cancer and these are found to be effective with minimal or no side effects. Objective: To evaluate the anticancer activity of polyherbal formulations (PGM-12 and PGM-13), Doxorubicin and combination of both in DMBA induced breast cancer in rats. Methodology: The mammary gland tumor in rats was induced by a single dose of 65mg/kg p.o of DMBA. After development of visible tumors, the rats were administered with polyherbal formulations (200mg/kg/p.o/day of PGM-12 and PGM-13), Doxorubicin (2.5mg/kg/i.p/ week) and combination of both polyherbal formulations and Doxorubicin for a period of 8 weeks. The anticancer activity of the polyherbal formulation was assessed by recording difference in tumor volume, tumor weight and histopathological examination of tumors after the study. To evaluate nephroprotective effect of treatment renal functional parameters and renal antioxidant enzymes were also measured. Results: The treatment with polyherbal formulations, Doxorubicin and combination showed a significant decrease in the tumor size and tumor weight compared to positive control. The polyherbal formulations has improved the renal function parameters and increased the antioxidant enzymes level significantly compared to positive control and doxorubicin group. Conclusion The findings of our study revealed that the combination of polyherbal formulation and doxorubicin exerted synergistic effect by inhibiting the tumor growth. The combination protected the kidney from the nephrotoxic effect of Doxorubicin in DMBA-induced mammary tumor in rats.