Background: Majority of scientific documentation suggested prominent role of Phytosterols towards Antidepressant activity. The main objective of the work was to evaluate Antidepressant activity of Holoptelea integrifolia isolated Phytosterol (HIIP) from petroleum ether extract (PEHI) of leaves of Holoptelea integrifolia (Roxb) Planch. Methods: The Antidepressant activity of different doses of HIIP (10 and 30 mg/kg-p.o.) was evaluated using Forced Swim Test (FST) in mice. Results: HIIP-30 mg/kg was more potent than HIIP-10 mg/kg for showing Antidepressant activity. Conclusions: The results indicate that HIIP shows Antidepressant activity which was dose dependent.