The aim of the present study was to investigate the effect of methanaloic leaf extract of Ventilago maderaspatana (MEVML, 200mg/kg b.w.) on oxidative stress markers in the diabetic rat liver. Diabetes exacerbates hepatic injury induced by STZ delivered free radical mediated oxidative stress. A marked decreased in the anti-oxidant marker enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and increase in malondialdehyde (MDA) was observed in the diabetic rats. Decreased activities of anti-oxidant enzymes in diabetic rats were augmented on oral administration of MEVML. Moreover, MEVML administration depleted the MDA level, which was earlier increased in the diabetic rats. These results suggest that MEVML exhibit a hepatoprotective effect by accelerating hepatic anti-oxidant defense mechanisms and down regulating the MDA levels to the normal levels in the diabetic rats. Thus, MEVML may be used as therapeutic agent in preventing complications in diabetic patients.