The human skin and soft tissue infections (SSTIs) caused by microbial pathogens during or after surgical procedures result in significant morbidity, prolonged hospitalization and huge economic burden[1]. The wide spread and inappropriate use of antibiotics were resulted in antibiotic resistance pathogens and leads to prolongation of illness, exacerbation of present disease, ineffective treatment, high risk of morbidity increased patient cost and patient harm[2]. The aim of this study was to assess the culture and sensitivity of microorganisms with various antimicrobials used in surgery department in a tertiary care hospital in India. This prospective observational study was carried out with 181 patients who had undergone various surgeries in the General Surgery department of Muthoot Healthcare Pvt Ltd, Kozhencherry, for a period of 6 months to assess the culture and sensitivity of microorganisms with various antimicrobials. In this study it was found that slough excision has most infection rate of 85.71%, followed by incision and drainage. Most of the cases found to be culture negative (71.11%) and only 28.88% was found to be culture positive. The most commonly isolated organism is S.aureus (8.88%) followed by E.coli (8.14%). On antimicrobial susceptibility testing, Piperacillin / Tazobactam was the most effective drug because it was sensitive to majority of the organism isolated. The second most effective drugs were Ceftriaxone and Cefpirome.