The presence of biologically active phytochemicals in medicinal plants contribute to their health benefits. A wide plant derived phytochemicals demonstrated bioactivity against diabetes and its complications. Our study concentrates on these bioactive compounds derived from Cyperus rotundus analysed through GCMS and docking them with the enzyme of polyol pathway Aldose Reductase (AR) responsible for diabetic retinopathy and other diabetic complications. AR inhibition studies have found that phytochemicals derived from plants be a good inhibitors of AR. The dichloro acetic acid (DCA) and Linoleic acid (9,12-Octadecadienoic acid (Z,Z) are identified bioactive compound showed a significant binding which indicates Cyperus rotundus may act as an inhibitor of enzyme and diabetic retinopathy.