Ivabradine is a heart rate lowering agent and controls the spontaneous diastolic depolarization in the sinus node to regulates heart rate. It is soluble in water, hydrochloric acid and in methanol. A simple, sensitive, precise and highly accurate UV spectrophotometric method has been developed for determination of Ivabradine HCL in formulated microsphere dosage form. Ivabradine HCl exhibited ? max at 286 nm in water, 0.1 N HCL solution and obeyed linearity in the concentration range of 20-100 mcg with Coefficient of determination value (R2) of 0.9960 . The slope, intercept, correlation coefficient, detection and quantitation limits were also calculated and found within statistical perimeter. Result of percentage recovery shows the method was not influenced by the presence of impurities or excipients. The method was validated by determining its sensitivity, accuracy and precision which proves suitability of the developed method for the routine estimation of Ivabradine in microsphere dosage form.