LC-UV method for quantification of Argatroban in human plasma has been developed and validated. Drug is extracted by protein precipitation technique using methanol. Separation has been performed on Phenomenex C8 column (150 Ã 4.6 mm, 5 ?m) with Phenomenex Security Guard Cartridges C8 (4x3mm) using acetonitrile: methanol: water (33:17:50) as mobile phase at 1.0 ml/min flow rate at 259 nm. The calibration curve was linear over concentration range of 200-5000 ?g/ml (r2=0.999). Argatroban was eluted at 4.2 min. The method is sensitive, precise and accurate. The method can be used for estimation of Argatroban in human plasma.
