Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents. Affinity chromatography is one of the most diverse and powerful chromatographic method for purification of a specific molecule or a group of molecules from a complex mixture by the specific interaction of solute with a ligand (which is immobilized). The affinity chromatography affords a means of characterizing interaction generated by an extremely broad range of binding affinities. In addition to its solute preparation and purification affinity chromatography thus also posess considerable potential for investigating the functional roles of the reactants there by purified. This review describes about the basic principle, procedure and its applications.
