The main objective of the present study is to synthesize a series of 2-substituted-4,5-dimethyl imidazoles ( IM1-IM6) by refluxing butan-2,3-dione with different substituted aldehydes in the presence of ammonium acetate and glacial acetic acid. The synthesized compounds IM1-IM6 was screened for antimicrobial activity. Out of 6 imidazole compounds IM3, IM4, IM6 exhibited moderate inhibition against gram negative bacterial species and especially against Escherichia coli while IM3, IM6 and IM4 showed maximum activity against most gram negative organisms. Against gram positive organisms almost all compound of the series exhibited maximum inhibition, especially IM2 and IM3 showed highest inhibition against B. megaterium. We can conclude that some compounds were exhibiting competent biological activity against both gram negative & gram positive bacterial species. Thus imidazole derivatives are having the significant anti microbial activity.
