In this present research work we developed a validated UV spectrometric method for Letrozole. It is simple, fast, accurate, cost efficient and reproducible spectrophotometric method, developed for the estimation of Letrozole in pure and pharmaceutical dosage form. Based on measurement of of absorption of UV light, the spectra of Letrozole in acetonitrile as a solvent showed maximum absorption wavelength (max) at 240 nm. The calibration curve was plotted over the concentration range from 1-24 ?g/ml of Letrozole with correlation coefficient 0.999. Validation was performed as per ICH Q2 guidelines for linearity, accuracy, precision, and recovery. This method has good reproducibility with % RSD less than one. The limit of detection (LOD) and limit of quantification were found to be 0.0470and 0.1424 respectively by simple UV spectroscopy. Thus proposed method can successfully applied for Letrozole in routine analysis work.
