Now a day those dosage form which can control the release rate. The microspheres are producing target drug delivery at specific time limit. Microspheres are making important part in novel drug delivery systems. The microspheres are typical free flowing powders consist of synthetic polymer which are biodegradable in nature and having particle size less than 200 um. The microspheres are made from highly transparent glass can perform as much high quality optical micro cavities or micro resonators. The success of these microspheres is limited having provided intimate contact of the drug delivery system with the absorbing membranes. The mucoadhesive microspheres are prepared by using different polymers like HPMC k-4, Ethyl Cellulose, Eudrajit L-100, chitosan, sodium alginate, calcium chloride, guar gum and xanthun gum etc. The microspheres drug delivery system are benefited in various dosage form like nasal drug delivery system, oral drug delivery system, transdermal drug delivery system, colonic delivery, gastro-intestinal drug delivery system, ophthalmic drug delivery system, gene delivery and intra-tumoral drug delivery system. The microspheres are widely accepted to achieve parentral and oral release. Drugs that are easily absorbed from the gastrointestinal tract (GIT) and have a short half-life are eliminated quickly from the blood circulation, so they require frequent dosing. These review focous on overview of different aspect of mucoadhesive microsphere, different preparation method of microsphere as well as evalution parameter and applications of mucoadhesive microsphere.