Abstract
The manuscript describes validated Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for the simultaneous estimation of Amlodipine Besylate and Indapamide in combined dosage form. The RP-HPLC separation was achieved on Intersil ODS C18 column (250 mm x 4.6 mm i.d., 5 µm particle size) at 40oC using mobile phase Acetonitrile : Acid Phthalate Buffer (pH 3.0 ± 0.05) (50 :50, v/v) at flow rate 1.0 mL/min. Retention time was found to be 2.31 and 5.11 for Amlodipine and Indapamide, Respectively. Quantification was achieved with photodiode array (PDA) detection at 240 nm over the concentration range of 10 - 50 µg/mL for Amlodipine Besylate and 3 - 15 µg/mL for Indapamide with mean recovery of 100.09 ± 0.52 % and 100.13 ± 0.09 % for Amlodipine Besylate and Indapamide, respectively. LOD and LOQ were found to be 0.69 µg/mL and 2.08 µg/mL for Amlodipine and 0.19 µg/mL and 0.58 µg/mL for Indapamide. These methods were found to be simple, sensitive, accurate, precise and economical and applicable for the simultaneous determination of Amlodipine Besylate and Indapamide in combined dosage form.
